Experiment/study

Overview

Users will be provided with a DOT kit including everything needed for the full analysis of one human mockup sample (HUMO). After performing this assay, the resulting measurements will be combined into a sample population study and published in a high impact journal. Participants will have the opportunity to be listed as co-authors on the final publication. Our goal is to accurately reconstruct the phenotypic statistics of the human population collectively and without any additional coordination.

The HUMOs provided in the kit are sampled from either a high or low phenotype in a double blind format. These phenotypes are defined by the number of target cells (HaCaT cell line, EpCAM+/EGFR+) that are dispersed amongst a much larger number of background PBMC cells. In the high phenotype samples targets are expected at the frequency of 1 in 10,000, while in the low phenotype samples the frequency is expected to be 1 in 100,000. The necessary sensitivity for this categorisation task lies outside the current capabilities of flow cytometry and enzymatic methods.

Tech Overview Distribution

Pictured In the figure are the simulated phenotypic statistics of the two HUMO samples. Each HUMO is sampled from a total of 107 cells and comprises 105 background PBMC cells with the number of target cells being phenotype-dependent (at frequencies of 10-4 and 10-5 for high and low phenotype, respectively). Total of 104 HUMOs per phenotype is collected. The distribution of target cells in randomly sampled HUMOs follows a binomial distribution. The reproducibility and sensitivity of DOT will be validated by plotting the reported results from each participant and comparing the experimental distribution to the theoretical one.

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